Table 3.
Extracted data from studies addressing blood specimen type. Where data were expressed as copies or haploid genome equivalents per mL, values were multiplied by 0.0033 ng to reach ng/mL. †, median values. *, values were estimated by reading off a graph, #, data for two different extraction methods were combined in the original paper.
| Author (et al) | Subjects (n) | Isolation method | Quantification method | Mean serum (ng/mL) | Mean plasma (ng/mL) | Ratio serum: plasma |
|---|---|---|---|---|---|---|
| Board | Healthy (10) | QIAamp® Virus Spin Kit | qPCR (AAT, 77 bp) | 24.65 | 5.07 | 4.86 |
| Chan | Healthy (27) | QIAamp® DBM Kit | qPCR (LEP, 105 bp) | 3.2* | 2.0* | 1.63 |
| Jung | Healthy (10) | NucleoSpin® Blood Kit | qPCR (HBB, 110 bp) | 8.3* | 4.0* | 2.08 |
| Kloten | Healthy (8) | Maxwell RSC | qPCR (KRAS) | 2300 | 450 | 4.18 |
| Cancer (50) | ccfDNA Plasma kit and QIAamp® CNA Kit | |||||
| Lee | Healthy (6) | HIV Monitor Assay | qPCR (HLA-DQA1, 242 bp) | 33* | 9.9* | 3.33 |
| Park | Cancer (130) | QIAamp® UltraSens Virus Kit | qPCR (ALU repeat, 81 bp) | 755.9 | 68.7 | 11.00 |
| Taback | Cancer (10) | QIAamp® Extraction Kit | PicoGreen® | 1059† | 259† | 4.09 |
| Thijssen | Healthy (28) | QIAquick® PCR Purification Kit | PicoGreen® | 12.9 | 4.8 | 2.69 |
| Cancer (26) | 47.6 | 10.6 | 4.49 | |||
| Umetani | Cancer (24) | None | qPCR (ALU repeat, 115 bp) | 970 | 180 | 5.39 |
| van Ginkel | Healthy (25) | QIAamp® CNA Kit and MagNA Pure LC DNA Isolation Kit | dPCR (BRAF, 91 bp)# | 673.2 | 60.72 | 11.09 |