Table 4.
Extracted data from studies addressing time-to-processing of blood. Stability is defined as the longest time point post-venepuncture in which cfDNA levels were not significantly elevated relative to at baseline (i.e. immediate processing of blood). All blood was stored at room temperature prior to processing unless indicated with a cross (†), indicating separate experiments were conducted on samples stored at both room temperature and 4 °C. d, days.
| Author (et al) | Subjects (n) | Isolation method | Quantification method | Tube type | Time points (hours) | Stability (hours) |
|---|---|---|---|---|---|---|
| Board | Healthy (10) | QIAamp® Virus Spin Kit | qPCR (AAT, 77 bp) | EDTA | 0, 2, 4, 8, 24 | 24 |
| Chan | Healthy (27) | QIAamp® DNA Blood Mini Kit | qPCR (LEP, 105 bp) | EDTA† | 0, 6, 24 | 6† |
| Garcia | Cancer (7) | QIAamp® Circulating Nucleic Acid Kit | qPCR (hTERT, 62 bp) | EDTA† | 4, 24 | 24 |
| Jung | Healthy (10) | NucleoSpin® Blood Kit | qPCR (HBB, 110 bp) | EDTA† | 0, 2, 4, 8 | 8† |
| Lam | Healthy (10) | QIAamp® Blood Kit | qPCR (HBB, 102 bp) | EDTA | 0, 2, 6, 24 | 6 |
| Lui | Healthy (8) | QIAamp® Blood Kit | qPCR (HBB, 102 bp) | EDTA | 0, 3, 6 | 6 |
| Page | Healthy (5) | QIAamp® DNA Blood Mini Kit | qPCR (GAPDH, 96 bp) | EDTA | 2, 6 | 6 |
| Denis | BRAFV600E-positive Melanoma (9) | iPrep™ PureLink® Virus Kit | therascreen®BRAF RGQ PCR Kit | EDTA | 2, 4d, 7d, 10d | 2 |
| Cell-Free DNA BCT® | 2, 4d, 7d, 10d | 10d | ||||
| Diaz | Healthy (60) | QIAamp® DNA Blood Mini Kit | qPCR (LINE-1, 96 bp) | Cell-Free DNA BCT® | 2, 3d, 5d | 5d |
| Kadam | Metastatic cancer (10) | QIAamp® DNA Blood Mini Kit | qPCR (ACTB, 182 bp) | EDTA | 0, 24 | 24 |
| NucleoSpin® Plasma XS Kit | Cell-Free DNA BCT® | 0, 24 | 24 | |||
| EDTA | 0, 24 | 0 | ||||
| Cell-Free DNA BCT® | 0, 24 | 0 | ||||
| Kang | Metastatic cancer (6) | QIAamp® Circulating Nucleic Acid Kit | dPCR (PIK3CA and TP53) | EDTA† | 0, 6, 48 | 6† |
| Cell-Free DNA BCT®† | 0, 6, 48 | 48† | ||||
| CellSave | 0, 6, 48 | 48 | ||||
| Markus | Healthy (23) | QIAamp® Circulating Nucleic Acid Kit | dPCR (9 single-copy loci) | Cell-Free DNA BCT®† | 24, 48 | 48 |
| Norton | Healthy (12) | QIAamp® Circulating Nucleic Acid Kit | qPCR (HBB, 136 bp) | EDTA | 0, 24, 48, 72, 7d, 14d | 24 |
| Cell-Free DNA BCT® | 0, 24, 48, 72, 7d, 14d | 7d | ||||
| Parpart-Li | Cancer (9) | QIAamp® Circulating Nucleic Acid Kit | dPCR (proprietary BRAF, EGFR and KRAS assays) | EDTA | 0, 24, 72, 5d, 7d | 0 |
| Cell-Free DNA BCT® | 0, 24, 72, 5d, 7d | 7d | ||||
| Rothwell | Healthy (20) | QIAamp® Circulating Nucleic Acid Kit | qPCR (RPPH1, 87 bp) | EDTA | 4, 4d | 4 |
| CellSave | 4, 4d | 4d | ||||
| Sherwood | Lung cancer (20) | QIAamp® Circulating Nucleic Acid Kit | qPCR (RPPH1, 87 bp) | EDTA | 2, 72 | 2 |
| Cell-Free DNA BCT® | 2, 72 | 2 | ||||
| Toro | Metastatic breast cancer (9) | QIAamp® Circulating Nucleic Acid Kit | dPCR (proprietary PIK3CA assays) | Cell-Free DNA BCT® | 24, 7d | 7d |
| PAXgene® Blood DNA Tube | 24, 7d | 24 | ||||
| van Dessel | Cancer (16) | QIAamp® Circulating Nucleic Acid Kit | qPCR (β actin, 136 bp) | EDTA | 1, 24, 96 | 24 |
| Cell-Free DNA BCT® | 1, 24, 96 | 96 | ||||
| CellSave | 1, 24, 96 | 96 | ||||
| van Ginkel | Healthy (6) | Quick-cfDNA™ serum & plasma Kit (Zymo Research) | dPCR (RRP30, 98 bp) | EDTA | 0, 3, 6, 24 | 24 |