Table 5.
Extracted data from studies addressing centrifugation speed and number of spins. ×2 indicates two centrifugations at the given speed, with removal of plasma from the cellular fraction or debris before the second centrifugation. *second centrifugation was conducted following freezing, then post-thawing of sample.
| Author (et al) | Subjects (n) | Isolation method | PCR assay | First speed (g) | Second speed (g) | Effect on cfDNA yield |
|---|---|---|---|---|---|---|
| Lui (a) | Healthy (14) | QIAamp® Blood Kit | HBB, 102 bp | 3,000 | 16,000 | No significance |
| (3,000) ×2 | 16,000 | |||||
| (3,000) ×2 | (16,000) ×2 | |||||
| Page | Healthy (5) | QIAamp® DNA Blood Mini Kit | GAPDH, 96 bp | 1,000 | 1,000 | No significance |
| 1,000 | 2,000 | |||||
| 1,000 | 10,000 | |||||
| Sherwood | Healthy (5) | QIAamp® Circulating Nucleic Acid Kit | RPPH1, 87 bp | 2,000 | None | No significance |
| 2,000 | 2,000 | |||||
| Lui (b) | Healthy (10) | QIAamp® Blood Kit | HBB, 102 bp | 400 | None | No significance |
| 800 | None | |||||
| 1,500 | None | |||||
| 3,000 | None | |||||
| 16,000 | None | |||||
| Swinkels | Healthy (10) | QIAamp® Blood Kit | HBB, 81 bp | 800 | None | Significantly lower yield after second spin (p = 0.01) |
| 800 | 16,000 | |||||
| van Ginkel | Healthy (6) | Quick-cfDNA™ serum & plasma Kit (Zymo Research) | dPCR (RRP30, 98 bp) | 800 | None | |
| 800 | 11,000 | No significance | ||||
| 800 | 11,000* |