Table 7.
Extracted data from studies comparing cfDNA quantitation method. *quantitative data not available.
| Author (et al) | Subjects (n) | Isolation method | Quantification methods | Results |
|---|---|---|---|---|
| Chiminqgi | Healthy (58) Colorectal (87) and prostate (93) cancer |
QIAamp® Blood Kit | PicoGreen® dsDNA Kit (qPCR HBB, 102 bp; PPIA, 105 bp) | PicoGreen correlated with qPCR targeting HBB (r = 0.915) and PPIA (r = 0.81). |
| Devonshire | Healthy (17) | QIAamp® Circulating Nucleic Acid Kit | qPCR and dPCR (ERV3, 135 bp; TERT, 79 bp, RPPH1, 64 bp) | Good agreement between the qPCR and droplet dPCR measurements of copy number* |
| Garcia | Cancer (7) | QIAamp® Circulating Nucleic Acid Kit | qPCR (hTERT, 62 bp), FL | Good agreement between qPCR and Qubit FL (r2 = 0.85; P < 0.00) |
| Mauger | Healthy (2) Colon (2), pancreatic (2), lung (2) and breast (2) cancers |
Norgen Plasma/Serum Circulating DNA Purification Mini Kit QIAamp® Circulating Nucleic Acid Kit |
Qubit® dsDNA HS assay; qPCR (DHFRP2, 102 bp; KPN, 102 bp) | Qubit did not correlate with qPCR assays targeting KPN (r = 0.04; P = 0.91) and DHFRP2 (r = 0.14; P = 0.70). Qubit correlated with qPCR assays targeting KPN (r = 0.75; P = 0.01) and DHFRP2 (r = 0.72; P = 0.02). |
| Ramachandran | Healthy (156) and prostate cancer (85) | QIAamp® UltraSens Virus Kit | PicoGreen® dsDNA Kit; qPCR (GSTP1, 95 bp); UV spectrophotometry | PicoGreen correlated with qPCR (r = 0.8552; P < 0.0001). UV absorbance weakly correlated with PicoGreen (r = 0.2376) and qPCR (r = 0.2274). |
| Szpechcinski | Lung cancer (10) | QIAamp® DNA Blood Midi Kit | PicoGreen® dsDNA Kit; qPCR (ACTB, 99 bp) by SYBR® Green and TaqMan® | PicoGreen correlated with SYBR® Green qPCR (r = 0.87; P < 0.0001) and TaqMan® qPCR (r = 0.94; P < 0.0001). SYBR® Green and TaqMan® qPCR were correlated (r = 0.96; P < 0.0001). |
| Thijssen | Colorectal cancer (26) | Isopropanol precipitation followed by QIAquick® PCR Purification Kit | PicoGreen®; dsDNA Kit qPCR (ALB, 81 bp) | PicoGreen correlated with qPCR (P < 0.05). |
| van Ginkel | Healthy (33 – Nanodrop) and Healthy (78 – Qubit) | Multiple commercial kits | dPCR vs Nanodrop and Qubit respectively | dPCR positively correlated with Qubit (r2 = 0.98; P < 0.0001). Nanodrop did not correlate with dPCR (r2 = 0.04; P = 0.81). |