FIGURE 1.

Study design (A), blood hemoglobin concentrations (B), quantification of intestinal copper absorption (C), and radioactive counts in blood (D), spleen (E), liver (F), kidney (G), heart (H), and bone (I) of male and female 7-wk-old anemic and nonanemic Dmt1^int/int and Dmt1^fl/fl mice (study 1). Results are depicted as box plots and represent n = 9 (serum hemoglobin concentrations), n = 8 (⁶⁴Cu absorption), n = 6 (⁶⁴Cu radioactivity in blood and spleen), n = 11 (in liver and heart), n = 7 (in kidney), or n = 10 (in bone) mice/group. No significant 3-way interactions were noted. Significant 2-way interactions and some main effects are denoted in each panel. Genotype main effects: P < 0.0001 (panels D, E, H, and I).*Slice test results (panels D, E, H, and I): normal, genotype not significant; IDA, genotype P < 0.0001. AdFe, adequate iron; cpm, counts per minute; Dmt1, divalent metal-ion transporter 1; Dmt1^fl/fl mice, phenotypically normal mice with the Slc11a2 gene “floxed”; Dmt1^int/int mice, intestine-specific Dmt1 knockout mice; Hb, hemoglobin; HFe, high iron; IDA, iron-deficiency anemia; LFe, low iron; Normal, normal hematologic variables.