. 2018 Aug 3;148(8):1244–1252. doi: 10.1093/jn/nxy111

TABLE 1.

Hematologic variables, BWs, and relative tissue weights of 3-mo-old male and female mice fed an adequate-iron diet with adequate copper content for 4 wk (study 2)¹

Sex, genotype	Hemoglobin, g/dL	Hematocrit, %	BW, g	Liver/BW, %	Kidney/BW, %	Heart/BW, %
Male, Dmt1^fl/fl	15.3 ± 1.0	59.5 ± 6.6	29.2 ± 3.7^a	4.07 ± 0.39	1.26 ± 0.14	0.47 ± 0.08^c
Male, Dmt1^int/int	1.5 ± 0.2	7.4 ± 1.7	19.4 ± 1.7^c	3.41 ± 0.26	1.58 ± 0.12	1.82 ± 0.28^a
Female, Dmt1^fl/fl	15.4 ± 0.8	60.4 ± 3.7	22.1 ± 2.3^b	3.71 ± 0.38	1.04 ± 0.06	0.45 ± 0.03^c
Female, Dmt1^int/int	1.8 ± 0.4	10.4 ± 2.9	21.1 ± 1.9^b,c	3.16 ± 0.27	1.31 ± 0.07	1.16 ± 0.33^b
Two-way interactions and main effects, P
Sex	0.30	0.10	0.0001	0.0009	<0.0001	<0.0001
Genotype	<0.0001	<0.0001	<0.0001	0.0001	<0.0001	<0.0001
Sex × genotype	0.39	0.36	<0.0001	0.52	0.82	<0.0001

Values are means ± SDs, n = 13–16/group. Data were analyzed by 2-factor ANOVA. Labeled means without a common superscript letter differ, P < 0.05. None of the physiological variables assessed here changed when mice were fed an adequate-iron diet with high copper content (data not shown). BW, body weight; Dmt1, divalent metal-ion transporter 1; Dmt1^fl/fl mice, phenotypically normal mice with the Slc11a2 gene “floxed”; Dmt1^int/int mice, intestine-specific Dmt1 knockout mice.