Figure 2.
Adult Nephrocyte RFP uptake. Human NS associated genes are listed with the corresponding Drosophila ortholog targeted by nephrocyte expression of inhibitory RNAi transgene. RFP fluorescence in nephrocytes was assessed in transgenic flies of genotype MHC-ANF-RFP; Hand-GFP; Dot-Gal4; UAS-GeneX-IR. ANF-RFP fusion protein uptake levels were determined from fluorescence micrographs and expressed relative to control nephrocytes of MHC-ANF-RFP; Hand-GFP; Dot-Gal4 transgenic flies. For quantification, ≥20 nephrocytes were analysed from each of three female flies per genotype. The results are presented as median plus interquartile range (IQR). Statistical significance (*) was defined as P < 0.05. Example fluorescence micrographs (merged GFP and RFP fluorescence) are shown to illustrate the range of effects on RFP levels induced by RNAi silencing of different genes.