Figure 3.

Adult Nephrocyte AgNO₃ uptake. Human NS associated genes are listed with the corresponding Drosophila ortholog targeted by nephrocyte expression of inhibitory RNAi transgene. AgNO₃ sequestration in nephrocytes was assessed in transgenic flies of genotype Dot-Gal4; UAS-Gene_X-IR. AgNO₃ levels were determined from photomicrographs and expressed relative to control nephrocytes in Dot-Gal4 transgenic flies. For quantification, ≥20 nephrocytes were analysed from each of three female flies per genotype. The results are presented as median plus interquartile range (IQR). Statistical significance (*) was defined as P < 0.05. Example photomicrographs are shown to illustrate the range of effects on AgNO₃ levels induced by RNAi silencing of different genes.