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Effect of doxorubicin (DOX) on follicle growth and survival during encapsulated in vitro follicle growth (eIVFG). A, Representative images of follicles treated with different doses of DOX on days 0, 4, and 8. B, Follicle diameters and C, follicle survival rates from days 0 to 8 after DOX exposure at 0–200 nM. Scale bar: 100 μm; error bar: standard deviation; *P < 0.05, **P < 0.01, and ***P < 0.001 compared to control group. N = 20–40 follicles for three replicates.
