Figure 3.

FlnA/B regulates FlnB/A expression in mouse long bone and actin stress fiber formation. Loss of FlnA and FlnB affects F-actin/stress fiber formation in ATDC5 cells. Compared to control cells, FlnA inhibition induces less F-actin formation whereas FlnB inhibition leads to more stress fiber formation following plating on fibronectin. Solid arrows in the fluorescent photomicrographs represent the non-stress fiber structure of F-actin, and open arrows represent stress fibers. Cultures were stained with phalloidin (rhodamine) and counterstained with Hoechst nuclear stain. The findings are quantitatively summarized in the graph below. Scale bars in A =50 μm. Quantification based on n > 50 cells.