Figure 2.

Knockdown of LINC00888 inhibited the cellular proliferation of melanoma. (A) LINC00888 expression in A375-S2 cells was detected by qRT-PCR after treatment with siRNA1, siRNA2, or siRNA3 for 72 hours. *p<0.05, **p<0.01. (B) The cell proliferation of A375-S2 after transfection with LINC00888 siRNAs or negative control for 24, 48, or 72 hours. *p<0.05, **p<0.01. (C) The apoptosis rate of A375-S2 cells with transfection of LINC00888 siRNAs (right panel) or negative control (left panel) was determined by Annexin V/PI staining in 72 hours. (D) The quantitative comparison of Annexin V/PI staining between positive cells and cells transfected with negative control or LIN00888 siRNA2 (n=3, **p<0.01 vs control). (E) Representative invasion assay readout of cells transfected with negative control or LIN00888 siRNA2 for 24 hours. (F) The cells which invaded through the membrane were fixed with paraformaldehyde, stained with crystal violet, and counted under an inverted microscope (normalized to control group, n=3, **p<0.01 vs control). (G) Protein expression of E-cadherin and vimentin in A375-S2 cells after transfection with LINC00888 siRNAs or negative control for 72 hours. (H) The quantification of E-cadherin and vimentin proteins expressions in the cells (n=3, *p<0.05, **p<0.01 vs control).

Abbreviations: PI, propidium iodide; qRT-PCR, quantitative real-time polymerase chain reaction.