Figure 3.

Overexpression of LINC00888 enhanced the A375-S2 cell proliferation and invasion. (A) Transfection of lenti-LINC00888 effectively overexpressed LINC00888 in the cells, revealed by qRT-PCR (n=3, **p<0.01 vs control). (B) The cell proliferation of cells transfected with lentivirus empty control or lenti-LINC00888 from 0 to 72 hours posttreatment (n=3, **p<0.01 vs control). (C) Representative invasion of cells transfected with negative control or lenti-LIN00888 was taken using a phase-contrast inverted microscope. (D) The quantitative comparison of invaded A375-S2 cells transfected with negative control or lenti-LIN00888 (n=3, **p<0.01 vs control). (E) Protein expression of E-cadherin and vimentin in A375-S2 cells after transfection with negative control or lenti-LIN00888 for 72 hours. (F) The quantification of E-cadherin and vimentin protein expression in the cells (n=3, **p<0.01 vs control). (G) A375-S2 cells were seeded in 6-well plates at 500 or 1,000 cells per well overnight, and then, the cells were treated with control or LINC00888 overexpression particles for 72 hours. And then, the pictures of colony format were taken using a digital camera. (H) The violet crystals were dissolved with 70% ethanol, and absorbance (595 nm) was measured with microplate reader (n=3, *p<0.05, **p<0.01 vs control).

Abbreviations: lenti-LINC0088, lentivirus LINC00888; qRT-PCR, quantitative real-time polymerase chain reaction.