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. 2018 Jul 31;11:4451–4459. doi: 10.2147/OTT.S167614

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© 2018 Zhou et al. This work is published and licensed by Dove Medical Press Limited

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Overexpression of miR-378 inhibited HCC cells proliferation in vitro.

Notes: (A) We transfected cells with miR-378 mimics and NC for 24, 48, and 72 h, and detected the transfection efficacy by real-time PCR. Data are expressed as mean ± SD, n=3, *P<0.05. (B) Growth curves of HepG2 and Hep3B cells after transfection with miR-378 mimics or NC were determined by CCK-8 assays. R Data are expressed as mean ± SD, n=3, *P<0.05. (C) Cell cycle assay showed that miR-378 caused G2/M arrest of HepG2 (8.7%–20.4%) and Hep3B (6.5%–17.9%) cells. Data are expressed as mean ± SD, n=3, *P<0.05.

Abbreviations: CCK-8, Cell Counting Kit-8; HCC, hepatocellular carcinoma; NC, negative control.