Figure 3.

AtA5 and myxothiazol (MXT) inhibit oxygen consumption and induce A3A-mediated RNA editing. (A) Graph depicts the relative fluorescence levels (mean and SEM with dashed lines), which reflect the degree of hypoxia, on treatment of monocyte-enriched PBMCs (MEPs) with AtA5 or MXT within approximately 3 h. Control indicates cells without any inhibitors. (B) Bar graph depicts L-Lactate levels in extra-cellular media from the samples analyzed in (A). NS: no significant (C) Bar graph depicts the percentage SDHB c.136 C > U RNA editing upon treatment of MEPs with MXT in normoxia or hypoxia (1 or 6% O₂) for 1 or 2 days. All panels show mean and SEM in MEPs from n = 3 donors.