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. 2017 Jun 20;26(18):3495–3507. doi: 10.1093/hmg/ddx235

Figure 1.

Figure 1

Knocking out Armc5 in mouse. (A) Gene targeting strategy. Armc5 exons are shown in black. In the targeting vector, LacZ and neomycin resistance gene (Neo) are inserted between the exons 1 and 2 of mouse Armc5 gene. The location of primers used for genotyping is shown along. (B) PCR analysis of Armc5 locus in Armc5+/+, Armc5+/− and Armc5−/− mice. The identity of the PCR products was confirmed by the size of the amplicon. (C) Only 0.025% of Armc5−/− embryos are still alive at weaning. The numbers of pups born from breeding between two Armc5+/− mice are presented in gray while the expected Mendelian proportions are in black. (D) Armc5+/+ and Armc5−/− males at weaning. Armc5−/− male is clearly smaller and lighter (10 g) than its littermate (18 g). The weight of Armc5+/+, Armc5+/− and Armc5−/− females (E) and males (F) was monitored over time. Armc5−/− females and males were significantly smaller than Armc5+/− and Armc5+/+ littermates even though they grew without any obvious defects.