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. 2017 Jun 20;26(18):3495–3507. doi: 10.1093/hmg/ddx235

Figure 6.

Figure 6

Heterozygosity for Armc5 deficiency leads to a hypocorticosteronemia at 12 months old. (A) Serum corticosterone concentration is significantly decreased in Armc5+/− mice at 12 months old but increased at 18 months. Bars represent the median corticosterone level of at least six individual adrenals ± standard deviation. (B) Serum ACTH concentration is significantly increased in 9 and 12 months old mice. Bars represent the mean ACTH level of at least six individual adrenals ± standard deviation. (C) star, Cyp11a1 and Cyp21 expression is significantly decreased in Armc5+/− adrenals. Mc2r, star, Cyp11a1, Hsd3b, Cyp21 and Cyp11b1 expression was analyzed by RTqPCR on mRNA from 12 and 18 months old Armc5+/+ and Armc5+/− adrenals. Bars represent the induction fold of at least six individual adrenals ± standard deviation. P-value was calculated using Student’s t-test. (D) Immunofluorescence for Cyp11a1 (a, c, d, green) and Akr1b7 (b, d, e, green) in adrenals from 18-month-old mice; DAPI stained the nuclei.