Table 2.

E. coli strains deposited at EUROSCARF (https://tinyurl.com/y8xxzpr9) and at Addgene (https://www.addgene.org) containing the single gRNA (pMEL) and double gRNA (pROS) plasmid series (Mans et al.2015).

Name	Accession no Euroscarf	Accession no Addgene	Relevant characteristics
p414-TEF1p-cas9-CYC1t		43802	ARS4 CEN6 bla TRP1 TEF1 p::Spcas9::CYC1t
pUG-natNT2		110922	bla AgTEF1p::nat::AgTEF1t
pMEL10	P30779	107916	2μm bla KlURA3 gRNA-CAN1.Y
pMEL11	P30780	107917	2μm bla amdSYM gRNA-CAN1.Y
pMEL12	P30781	107918	2μm bla hphNT1 gRNA-CAN1.Y
pMEL13	P30782	107919	2μm bla kanMX gRNA-CAN1.Y
pMEL14	P30783	107920	2μm bla KlLEU2 gRNA-CAN1.Y
pMEL15	P30784	107921	2μm bla natNT2 gRNA-CAN1.Y
pMEL16	P30785	107922	2μm bla HIS3 gRNA-CAN1.Y
pMEL17	P30786	107923	2μm bla TRP1 gRNA-CAN1.Y
pROS10	P30787	107924	2μm bla URA3 gRNA-CAN1.Y gRNA-ADE2.Y
pROS11	P30788	107925	2μm bla amdSYM gRNA-CAN1.Y gRNA-ADE2.Y
pROS12	P30789	107926	2μm bla hphNT1 gRNA-CAN1.Y gRNA-ADE2.Y
pROS13	P30790	107927	2μm bla kanMX gRNA-CAN1.Y gRNA-ADE2.Y
pROS14	P30791	107928	2μm bla KlLEU2 gRNA-CAN1.Y gRNA-ADE2.Y
pROS15	P30792	107929	2μm bla natNT2 gRNA-CAN1.Y gRNA-ADE2.Y
pROS16	P30793	107930	2μm bla HIS3 gRNA-CAN1.Y gRNA-ADE2.Y
pROS17	P30794	107931	2μm bla TRP1 gRNA-CAN1.Y gRNA-ADE2.Y