| Component | Amount (μL) |
|---|---|
| 5x Phusion HF buffer | 10 |
| dNTPs (10 mM) | 1 |
| pMEL template* | 1–5 ng |
| Primer 6005 (10 μM) | 1 |
| Primer 6006 (10 μM) | 1 |
| Phusion polymerase | 0.75 |
| MilliQ | X |
| Total volume | 50 |
*
This can be any pMEL plasmid
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.