| Mixture 1 | |
|---|---|
| Component | Amount (μL) |
| DreamTaq Master Mix (2x) | 5 |
| Template | * |
| Primer gRNAt 1 (10 μM) | 0.5 |
| Primer α (10 μM) | 0.25 |
| Primer β (10 μM) | 0.25 |
| MilliQ | 4 |
| Total volume | 10 |
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