FIG. 1.

Effect of DEHP on the exposed dams. A, Time-mated C57Bl/6J dams were dosed via oral gavage once a day with 0, 5, 250, and 500 mg/kg of body weight of DEHP in corn oil from E7.5 to E16.5. Survival rate, gross morphology, anogenital distance (AGD), and testis parameters of the fetuses were examined on E18.5. Some dams were sacrificed at E14.5 for analyses of gene expression in the fetal testes. B, Weight (g) of the dams over the exposure period. One-way ANOVA followed by Dunnet’s multiple comparison test were used for statistical analyses. The weight of the treated dams was compared with the control group at each time point. *P < 0.05, ** P < 0.01, *** P < 0.001. C, Weight of the E18.5 fetuses at the time of collection. The weight of the DEHP exposed fetuses was compared with the control group for each sex. Statistical significance was analyzed using Dunnett’s test coupled with mixed effects analysis of variance to control for the litter effect. * P < 0.05, ** P < 0.01.