Figure 2—

MR is reduced during sleep state. Female control flies (w¹¹¹⁸) were allowed to acclimate in the system for 24 hours. (A) MR shows an inverse pattern to their sleep (N = 24). (B) Total minutes of sleep per 12 hours of day and night for B (N = 24; p < .001). (C) The MR of female flies as CO₂ produced per hour, over 24 hours of day and night (N = 24; p < .002). (D) The MR throughout a single, representative sleep bout during the night. (E) Linear regression model comparing percent change in MR versus sleep duration, binned per 5 minutes (N = 24; R² = 0.266). Gray dashed lines indicate 95% confidence interval. One-way ANOVA comparing the initial percent change in MR at the 10-minute sleep bin to each subsequent sleep bin reveals significant differences after 35 minutes asleep (N = 24 each sleep bin; p < .05). ANOVA = analysis of variance.