Figure 4—

Reduced MR during pharmacologically induced sleep. (A) Female w¹¹¹⁸ flies were loaded on sucrose or sucrose containing 0.1 mg/mL gaboxadol 2 hours before lights out (ZT10), acclimated to the system for 12 hours during the night phase and were measured for 12 hours (ZT0-12) during the following day. (B) Daytime sleep was significantly elevated in gaboxadol-treated flies (green) compared to flies fed sucrose alone (black). (C) Quantification of total sleep reveals gaboxadol-treated flies (N = 15) sleep significantly longer than untreated controls (N = 14; p < .0001). (D) MR was reduced throughout the 12-hour day. (E) Quantification of mean MR reveals a significant reduction in gaboxadol-treated flies (N = 15) compared to controls (N = 14; p < .0001). (F) Linear regression of percent change in MR versus sleep duration, binned per 5 minutes (N = 15; R² = 0.200). Gray dashed lines indicate 95% confidence interval. One-way ANOVA comparing the initial percent change in MR at the 10-minute sleep bin to each subsequent sleep bin reveals significant differences after 30 minutes asleep. (N = 15 each sleep bin; p < .05). ANOVA = analysis of variance.