Figure 6—

Sleep-dependent changes in metabolism are intact in trsn^null flies. (A) Sleep did not significantly differ between trsn^null flies housed on sucrose or starved on agar alone. (B) Quantification of total nighttime sleep (ZT12-ZT24) revealed sleep is significantly lower in w¹¹¹⁸ control flies (N = 30) housed on agar compared to fed (N = 28; p < .0001), while there is no significant difference between trsn^null flies (N = 28) housed on 5% sucrose or agar alone (N = 25; p > .05; two-way ANOVA F_(1,107) = 42.52, p < .0001). Treatment-by-genotype interaction is significant (two-way ANOVA F_(1,107) = 11.24), p < .01). (C) MR is lower in control and trsn^null flies housed on agar compared to flies housed on 5% sucrose. (D) Quantification revealed MR is lower in both starved trsn^null flies and controls (w¹¹¹⁸, p < .0001; trsn^null, p < .01; two-way ANOVA F_(1,107) = 28.22, p < .0001). There is no effect of treatment-by-genotype interaction (two-way ANOVA F_(1,107) = 0.7162, p > .30). (E) Applied linear regression model comparing percent change in MR versus sleep duration, binned per 5 minutes reveals a correlation in w¹¹¹⁸ fed flies (N = 28; R² = 0.208), but only a weak effect in w¹¹¹⁸ starved flies (N = 25, 5 flies did not sleep on agar; R² = 0.097). Gray dashed lines indicate 95% confidence interval of each line. Comparison of the regression lines indicate that the slopes are different between the w¹¹¹⁸ fed versus starved state (F = 7.09725, p< .01). One-way ANOVA comparing the initial percent change in MR at the 10-minute sleep bin to each subsequent sleep bin within each group reveals significant differences after 40 minutes asleep in fed flies (N = 28 each sleep bin; p < .05) and differences in starved flies beyond 55 minutes (N = 25 each sleep bin; p < .05). (F) Regression analysis model comparing percent change in MR versus sleep duration, binned per 5 minutes reveals a correlation in trsn^null fed flies (N = 28; R² = 0.201), but only a weak effect in trsn^null starved flies (N = 25; R² = 0.183). Gray dashed lines indicate 95% confidence interval of each line. Comparison of the regression lines indicates that the slopes do not differ between the trsn^null fed versus starved state (F = 5.0557, p < .05). One-way ANOVA comparing the initial percent change in MR at the 10-minute sleep bin to each subsequent sleep bin within each group reveals significant differences after 25 minutes asleep in trsn^null fed flies (N = 28 each sleep bin; p < .05) and differences in trsn^null starved flies beyond 30 minutes (N = 25 each sleep bin; p < .05). ANOVA = analysis of variance.