Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 1;32(13-14):929–943. doi: 10.1101/gad.313932.118

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Zhang et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Figure 6. — Loss of Smcr8 results in down-regulation of lysosomal lumen protein expression. (A) Differentially expressed proteins in Smcr8^−/− BMDMs compared with wild-type BMDMs by quantitative mass spectrometry. Data are represented as log₂ of protein expression fold change in Smcr8^−/− versus Smcr8^+/+ on the X-axis and log₁₀ of adjusted P-value by moderated t-test on the Y-axis. (Orange diamond) Smcr8; (blue dots) lysosome-located genes; (red dots) proteins that can be found in lysosomes, extracellular space, and extracellular exosomes. (B) Summary of gene ontology cellular component enrichment analysis of differentially expressed proteins in Smcr8^−/− BMDMs. (C) Top biological processes that are enriched in the list of differentially expressed proteins in Smcr8^−/− BMDMs. (D) Secreted cathepsin B (CtsB) in conditioned medium, total CtsB, and total Gapdh as a loading control in Smcr8^+/+ and Smcr8^−/− BMDMs. (E) Quantification of secreted CtsB levels in the BMDM conditioned medium. (*) P < 0.05 by two-tailed Student's t-test.