Figure 2.

SYP51 is found on the TGN and PVC. Cryosections of Arabidopsis root cells were immunolabeled with either SYP51 preimmune (A), affinity-purified SYP51 antibodies (B–D) followed by detection with 10 nm of colloidal gold. Double immunolabeling was performed with the use of cryosections from wild-type (E–H), or HA-SYP41 and T7-SYP42-expressing (I–M) roots. In all cases, SYP51 (or its preimmune) immunolabeling was detected with 10 nm of gold, whereas the second antibody was detected with 5 nm of gold (as indicated in the lower right on each panel). SYP51 preimmune (E and I) or affinity-purified antibodies (G and H, J–M, and insets) were detected with 10 nm of gold. A second labeling was then preformed with SYP21 preimmune (E and F) or affinity-purified antibodies (G and H, and inset), with a nonspecific control (I), T7-monoclonal antibodies (J and K), or HA-monoclonal antibodies (L and M), each detected with 5 nm of gold. All images were captured at the same magnification. G, Golgi; V, vacuole. Bar in A, 100 nm. Arrowheads are used to highlight the 5-nm gold particles.