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. 2018 Aug 1;32(15-16):1085–1100. doi: 10.1101/gad.314096.118

Figure 3.

Figure 3.

Genetic interaction among PLT, TCP, and SCR during embryogenesis. (A) The wild-type (Col-0) and plt1-3−/−plt3-1−/−tcp20-1−/−scr-3−/− embryos at the dermatogen to globular stages. Original cleared images (left) and merged images with tracings of embryos (right) are shown. Bars, 20 µm. (B) Expression patterns of PLT3-YFP, SCR-YFP, TCP20-YFP, and pWOX5::H2B-YFP at the transition between the dermatogen and early globular stages (left and middle) and expression heat maps during the late globular/transition states (right) are shown. Bars, 10 µm. (C) YFP signal intensities of PLT1-YFP, PLT3-YFP, SCR-YFP, TCP20-YFP, TCP21-YFP, and pWOX5::H2B-YFP during the octant to transition/heart stages exemplified in B. Box length represents the range in which the central 50% of the values fall, with the box edges at the lower (orange) and upper (gray) quartiles. The whiskers indicate the highest and lowest values. YFP fluorescence intensities (n > 15) in the QC (or its precursor cells in the octant and dermatogen stages) were quantified using ImageJ. (D) Cellular anatomies of the radicle in wild-type and scr-3−/−, plt1-1−/−tcp20-1−/−scr-3−/−, plt3-1−/−tcp20-1−/−scr-3−/−, tcp20-1−/−scr-3−/−, and plt1-3−/−plt3-1−/−tcp20-1−/−scr-3−/− homozygous mature embryos. The numbers of embryos that showed improper cell divisions in the QC per examined total embryos are indicated in the respective panels. (Yellow arrows) Position of the QC; (dots) positions of the columella cell layers. Bars, 30 µm.