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. 2018 Aug 1;32(15-16):1035–1044. doi: 10.1101/gad.312355.118

Figure 2.

Figure 2.

Loss of PPARγ reduces macrophage respiration and blocks the effects of Rosi. (A) Macrophage respiration as measured by OCR in DMEM containing 10 mM glucose and 2 mM glutamine in control and MPKO macrophages after 24 h of stimulation with 1 µM Rosi or DMSO vehicle control. During measurements, macrophages were treated with the complex V inhibitor oligomycin (O), the uncoupler fluoro-carbonyl cyanide phenylhyrazone (FCCP) (F), and the complex I and III inhibitors rotenone/antimycin A (R/A) at the times indicated (gray arrows). (B) Basal and maximal respiration and oxygen consumption from ATP production calculated from OCRs in control and MPKO macrophages. Calculations were corrected for nonmitochondrial respiration. Data are representative of at least three independent experiments. Data points are represented as mean ± SEM from n = 6–12 technical replicates. (*) P < 0.05.