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. 2017 May 13;26(16):3081–3093. doi: 10.1093/hmg/ddx192

Figure 3.

Figure 3

Measurement of ROS production in the GNE myopathy muscle in vivo using microdialysis. (A-B) Experimental set up and representative HPLC chromatograms. Perfusion medium containing 5 mM salicylate (Sal) was pumped through a microdialysis probe and DHBA in dialysate was detected by HPLC–electrochemical detection system: (A) baseline, (B) post-stimulus. (C) Electrical stimulus (ES) consisted of 50V, 40 Hz, 3 ms pulses for 300 trains. HPLC profils of DHBA during the experiments. (D) DHBA increments after contraction in GNE myopathy mice (n = 9) and littermates (n = 5). Pre-ES, before ES. ES1, 1st 20 min after ES. ES2, 2nd 20 min after ES. Data represent mean ± SEM. Each circle represents a DHBA level from an individual mouse. *P < 0.05.