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. 2001 Dec;12(12):3744–3758. doi: 10.1091/mbc.12.12.3744

Figure 5.

Figure 5

Rad1-ICD and Rad1-P279 mutant proteins interact efficiently with Hus1 and Rad9. Western blots of IPs from fission yeast protein extracts probed with anti-Hus1, anti-Rad9, or anti-Rad1 antibody. (A) Anti-Rad1 IPs from rad1Δ cells expressing pREP1 plasmid (pTE102, lane 1), pREP1-rad1-ICD (pTE885, lane 2), pREP1-rad1-P279A (pTE886, lane 3), and pREP1-rad1+ (pTE567, lane 4) immunoblotted with anti-Hus1 (top), anti-Rad9 (middle), or anti-Rad1 antibody (bottom). (B) Anti-Rad9 IPs from rad1Δ cells expressing the same plasmids as mentioned above probed with anti-Hus1 (top), anti-Rad9 (middle), or anti-Rad1 antibody (bottom).