Skip to main content
. 2001 Dec;12(12):3744–3758. doi: 10.1091/mbc.12.12.3744

Figure 6.

Figure 6

Hus1–Rad1–Rad9 interactions are disrupted in the checkpoint defective rad1-S3 and rad1-S4 mutants. IPs were performed on fission yeast extracts with the use of anti-Rad1, anti-Hus1, or anti-Rad9 antibody. Immunoprecipitates were analyzed for the presence of Hus1, Rad9, or Rad1 from wt cells (TE237, lane 1), rad1-S3 (TE994, lane 2), rad1-S4 (TE995, lane 3), rad1-S5 (TE992, lane 4), and rad1-S6 (TE993, lane 5) mutants. (A) Western blot of anti-Rad1 IPs probed with anti-Hus1 (top), anti-Rad9 (middle), or anti-Rad1 antibody (bottom). (B) Western blot of anti-Rad9 IPs probed with anti-Hus1 (top), anti-Rad1 (middle), or anti-Rad9 antibody (bottom). (C) Western blot of anti-Hus1 IPs probed with anti-Rad1 (top), anti-Rad9 antibody (middle), or anti-Hus1 antibody (bottom).