Table 1.
Primer and Probe Sequences Used for Quantitative Real-Time PCR Analyses.
| Gene Symbol | Amplicon (bp) | Forward (F) and reverse (R) primers and probe (P) sequence |
|---|---|---|
| Arc | 68 | F: GCAGGTGGGTGGCTCTGA |
| R: TCTTGGCTGGCCCATTCA | ||
| P: 6FAM5′-AATATTGGCTGTCCCAGATCCAGAACCAC-3′TAMRA | ||
| Egr1 | 66 | F: AAGACACCCCCCCATGAAC |
| R: GCGAGAAAAGCGGCGAT | ||
| P: 6FAM5′-CCCATATGCTTGCCCTGTCGAGTCC-3′TAMRA | ||
| Grp78 | 59 | F: CACGTCCAACCCCGAGAA |
| R: ATTCCAAGTGCGTCCGATG | ||
| P: 5′FAM-CGGTCTTCGATGCCAAGCGCC-3′TAMRA | ||
| Grp94 | 64 | F: CTTCCTTGTAGCAGATAAGGTCATTG |
| P: 5′FAM-CACATCGAAACACAACAATGATACCCAGCA-3′TAMRA | ||
| R: TCATTGGAGTCTGATTCCCAGA | ||
| Nr4a3 | 65 | F: CCTCCGATCTGTATGATGAATGC |
| R: GTGGGACAGTATCTGGAATAATCAAG | ||
| P: 5′FAM-CGAGCTTTAACAGATGCAACACCCAGAGA-3′TAMRA | ||
| Fra2 | 90 | F: TCTTCCTCAGCAGGGATGGA |
| R: CAGAGGCTCTTCCCCGTAGA | ||
| P: 6FAM5′-CCAGCGCTCTGTCATCAAGCCCAT-3′TAMRA | ||
| Egr3 | 68 | F: TTGCTAAATCAATTGCCTGACAA |
| R: CCGGAGAAGAGGTTGAGCG | ||
| P: 6FAM5′-CTGTACCCCGAGGAGATCCCCAGC-3′TAMRA | ||
| Egr2 | 111 | F: GGACCCAGGTCTCATTCCTATG |
| R: GGACAGGGAAACGGCTTTC | ||
| P: 6FAM5′-ATCCTGGATTTTTTCCATCTCCGTGCC-3′TAMRA | ||
| Egr4 | 79 | F: TACAGCGGCAGCTTCTTCATC |
| R: TGCCAGACATGAGGTTGAAGAG | ||
| P: 6FAM5′-TTCCCGAACACCCGCACGACC-3′TAMRA | ||
| Mmp9 | 62 | F: ACCCGAAGCGGACATTGTC |
| R: CGAAGGGATACCCGTCTCC | ||
| P: 6FAM5′-TCCAGTTTGGTGTCGCGG-3′TAMRA | ||
| Tpa | 93 | F: CAACAGCGGCCTGGTACAAT |
| R: TACAGGGCCTGCTGACACGT | ||
| P: 6FAM5′-TGCCTGTCCGAAGTTGCAGCGAAC-3′TAMRA | ||
| Camk2 | 88 | F: TCCTGCCGCATTGAAGGA |
| R: AACCAGCAGCCACATTCCA | ||
| P: 5′FAM-CTTGCTTCGCAGAGATCCGCTCTTTG-TAMRA-3′ | ||
| Homer | 120 | F: TTGACCCGAACACAAAGAAGAA |
| R: TATTGCCTTTGAGCCATCTAAACTG | ||
| P: 5′FAM-CCCACCAGCAAGCATGCAGTTACTGTATC-TAMRA-3′ | ||
| Narp | 134 | F: GGCAAGCCAACGAGATTGTG |
| R: AGTGGTCCAGGTGATGCAGAT | ||
| P: 5′FAM-CTGCTCATCAACGACAAGGTCGCACA-TAMRA-3′ | ||
| Neurogranin | 91 | F: GGCCAGAGCTGAACGTTTTAGA |
| R: GCTCACAAACACAGTAGGGAAGTC | ||
| P: 5′FAM-CGCGTCCCCTTCGCAGTGACA-TAMRA-3′ | ||
| Scg2 | 84 | F: TTTAATGCCCAATTTCCCTTCTT |
| R: CACTGTCAACAGGATAGAGATCAACA | ||
| P: 5′FAM-CCCCCAAGTAAGCCCCCTACATTTCTCT-TAMRA-3′ | ||
| Syt4 | 105 | F: GACAGAGCACGCAGAAAACATG |
| R: GTGAAGACGAGGCCAAAAGC | ||
| P: 5′FAM-CTCCTATCACCACCAGCCGCGTG-TAMRA-3′ | ||
| Bdnf | 68 | F: CCATAAGGACGCGGACTTGTAC |
| R: GAGGAGGCTCCAAAGGCACTT | ||
| P: 5′FAM-CTTCCCGGGTGATGCTCAGCAGT-TAMRA-3′ | ||
| G3pdh | 65 | F: CAACGGGAAGCCCATCAC |
| R: CGGCCTCACCCCATTTG | ||
| P: 6VIC5′-ATCTTCCAGGAGCGAGACCCCACTAACA-TAMRA-3′ | ||
| hsa-miR-132 | Applied Biosystems, Catalog Number 4373143 | |
| mmu-miR-134 | Applied Biosystems, Catalog Number 4373299 |