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Oxygen consumption rates in fibroblasts. Cellular oxygen consumption rates (OCR) for fibroblast cell lines from wild-type and TRAK1 c.287-2 A > C patient (Patient A-IV.3) was measured by XF24 extracellular flux analysis. Oligomycin (1.25 µM), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, 0.2 µM), and antimycin A (1.8 µM) together with rotenone (1 µM) were injected after 20, 50 and 80 min, respectively. n = 4 plates; *P-value < 0.05.
