Table 1.
Antibodies used in the study
| Antibody | Immunogen | Host | Dilution | Source | Catalog number |
|---|---|---|---|---|---|
| Tau-1 | Bovine microtubule associated protein | Mouse | 1:200 | Cell signaling | 4019 |
| AT-180 | Peptide containing phospho-T231/S235 | Mouse | 1:200 | Thermo | P10636 |
| AT-270 | Peptide containing phospho-T181 | Mouse | 1:300 | Thermo | MN1050 |
| PHF-13 | Peptide containing phospho-Ser396 | Mouse | 1:300 | Cell signaling | 9632 |
| PHF-1 | Peptide containing phosphor-Ser396/S404 | Mouse | 1:200 | Dr Davies P. | N/A |
| PSD95 | Purified recombinant rat PSD-95 | Mouse | 1:200 | Thermo | MA1-045 |
| SYP | aa 221–313 of SYP of human origin | Mouse | 1:400 | Santa Cruz | sc-55507 |
| MAP2 | Purified microtubule-associated protein from rat brain | Rabbit | 1:300 | Millipore | AB5622 |
| GFAP | Spinal cord homogenate of bovine origin | Mouse | 1:300 | Santa Cruz | sc-33673 |
| CD45 | aa 1075–1304 of CD45 of human origin | Rabbit | 1:200 | Santa Cruz | sc-25590 |
| Actin | Gizzard Actin of avian origin | Mouse | 1:1000 | Santa Cruz | sc-47778 |