Figure 5.

Higher frequency of CD11b⁺macrophages and reduction of the yolk sac-derived population in SMA spleens. Total splenocytes from individual WT (left) and SMA (right) spleens of SMNΔ7 mice at PND 12 were surface labeled. (A) Frequency of CD11b macrophages (CD3^-, B220^-, CD11b⁺) are indicated relative to live CD3^-, B220^- splenocytes in representative FACS plots. (B) Bar graph showing the mean percentages ± SEM for six mice per group. (C) Total splenocytes from individual WT (upper) and SMA (lower) spleens were stained with anti-CD11b and anti-F4/80 antibodies and the different subsets of macrophages were identified by flow cytometry. Frequency of (F4/80^low CD11b^high) and (F4/80^high CD11b^low) subsets are indicated relative to live CD45⁺splenocytes in representative FACS plots. Right panels depict the percentage of cells expressing CX3CR1 in each of the two macrophage populations. (D) Bar graph showing the mean percentages ± SEM for six mice per group. (E,F) Same as (C) and (D) for spleens harvested at PND 2.