Skip to main content
. 2017 Apr 7;26(12):2321–2334. doi: 10.1093/hmg/ddx125

Figure 7.

Figure 7

M1-C448Y spastin alters mitochondrial distribution in a CK2-dependent manner. (A–D) Analysis of mitochondrial distribution in RFL-6 rat fibroblasts under various experimental conditions, while (F–I) are the same experimental conditions but with primary rat cortical neurons. (A,F) are cells expressing M87-C448Y under treatment with DMSO, the vehicle-control. (B,G) are cells expressing M87-C448Y under treatment with TBCA. (C,H) are cells expressing M1-C448Y under treatment with DMSO, the vehicle-control. (D,I) are cells expressing M87-C448Y under treatment with TBCA. In both cell types, M1-C448Y caused mitochondrial dispersion, but M87-C448Y did not. TBCA had no effect on mitochondrial distribution in cells expressing M87-C448Y, but reverted the mitochondrial dispersion back to the control pattern in cells expressing M81-C448Y. Quantitative data are shown for RFL-6 fibroblasts (E) and neurons (J). See Results for numerical data. *P < 0.05. Bar, 10 microns.