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. 2017 Mar 13;26(10):1890–1899. doi: 10.1093/hmg/ddx097

Figure 4.

Figure 4

 Effects of EX-527 on AMPK/STAT3/NFκB signaling in wild-type and AAV-NT mice. Wild-type and AAV-NT mice at age 64–74 weeks were treated daily for four weeks with EX-527 and their phenotype analyzed at age 68–78 weeks. For quantitative RT-PCR, the data were analyzed for wild-type (+/+, n = 8), wild-type-EX-527 (+/+/EX-527, n = 8), AAV-NT (n = 8) and AAV-NT-EX-527 (n = 8) mice. For densitometry analysis, the data were analyzed from 4 pairs of wild-type/AAV-NT and wild-type-EX-527/AAV-NT-EX-527 mice. (A) Body weight, body fat and fasting blood glucose levels of mice before and after EX-527 treatment. (B) Western blot analysis of p-AMPK-T172, AMPK and β-actin with quantification of protein levels by densitometry. (C) Western-blot analysis of p-STAT3-Y705, STAT3, NFκB-p65, Ac-NFκB-p65-K310 and β-actin and quantification protein levels by densitometry. Data represent the mean ± SEM; *P < 0.05; **P < 0.005.