Table 2. The Effect of H2S donors on expression of Senescence-Associated Secretory Phenotype (SASP) proteins.

	Control	Young Cells	AP39	AP123	RT01	GYY
GM-CSF	2.915 (0.241)	3.444 (0.243)	3.008 (0.391)	3.659 (0.213)	3.651 (0.159)	10.410 (0.455)***
INFg	41.099 (3.267)	41.439 (2.984)	38.346 (3.727)	29.774 (5.240)	45.805 (7.065)	40.817 (3.337)
IL10	0.299 (0.059)	0.412 (0.061)	0.441 (0.069)	0.387 (0.061)	0.477 (0.067)	0.329 (0.034)
IL12p70	0.797 (0.173)	0.832 (0.169)	0.561 (0.145)	0.959 (0.314)	0.811 (0.245)	0.515 (0.052)**
IL1b	0.317 (0.182)	0.176 (0.039)	0.172 (0.047)	0.283 (0.054)	0.217 (0.074)	0.280 (0.044)
IL2	0.713 (0.268)	1.008 (0.583)	1.130 (0.287)	0.449 (0.167)	0.980 (0.230)	1.487 (0.186)***
IL6	1431.728 (11.463)	1433.852 (12.712)	1439.876 (46.458)	1454.688 (41.138)	1454.689 (41.138)	710 (35.799)***
IL8	5885.113 (349.38)	6750.428 (156.941)***	7342.547 (295.224)***	7461.439 (210.010)***	7559.555 (327.008)***	17321.310 (631.756)***
TNFa	4.592 (0.512)	5.463 (0.713)	4.974 (0.582)	4.489 (1.124)	5.211 (0.602)	4.443 (0.059)

Protein levels of components of the senescence associate secretory phenotype (SASP) were determined using Mesoscale ELISA platform in the culture medium from senescent endothelial cells treated with Na-GYY4137 (at 100ug/ml) or AP39, AP123, RT01 (at 10ng/ml) for 24 hours and from an early passage (Young cells) PD24. The results are expressed as pg/ml of 3 independent experiments. Control refers to vehicle-only treated cells. Standard Deviation (SD) is given in parentheses. Statistically-significant findings are indicated in bold typeface, and significance level is indicated by stars with **p<0.005 and ***p<0.001 compared with the corresponding control value (senescent endothelial cells).