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. 2016 Sep 3;27(7):3736–3751. doi: 10.1093/cercor/bhw194

Figure 1.

Figure 1.

HIF-1α expression in the human fetal forebrain at 18–22 gw. (A) Partial view of a coronal section through the telencephalon at 18 gw, after HIF-1α in situ hybridization. (B–D) HIF-1α mRNA expression in the CP (B, B′), cortical ventricular zone (CX-VZ; C, C′), and GE VZ (D, D′). Arrowheads indicate HIF-1α expression in RGCs. (E) HIF-1α mRNA expression levels in tissue and sorted RGCs from the cortex and GE. (F–H) Immunohistochemistry for HIF-1α (green) and GFAP (red) in the cortex and GE (F–H, F′–H′). Insets show higher magnifications of the boxed areas. White arrowheads indicate HIF-1α expression in GFAP+ RGCs in the VZ of both the GE and CX. (I) Z-projection image showing a GFAP+ RGC expressing HIF-1α in the cortical VZ. Abbreviations: CP, cortical plate; CX, cortex; GE, ganglionic eminence; IZ, intermediate zone; LV, lateral ventricle; VZ, ventricular zone; SP, subplate; SVZ, subventricular zone. Scale bars: 20 µm. Significant difference: *P < 0.05 in a t-test. Error bars indicate the standard error of the mean (SEM).