Table 3.
Antibodies used in this study
| Antigen | Host | Clone | Manufacturer | Catalog no. | Method |
|---|---|---|---|---|---|
| ASCL1 | Mouse IgG1 | 24B72D11.1 | BD Pharmingen | 556 604 | WB |
| β-III-tubulin | Mouse polyclonal | Dako | PRB-435P | ICC | |
| β-Actin | Mouse IgG | Thermo Scientific | MA5-15739 | WB | |
| β-Catenin | Mouse IgG1 | 14/β-Catenin | Becton-Dickinson | 610 153 | IHC, WB |
| BLBP | Rabbit IgG | Abcam | ab27171 | ICC | |
| BrdU | Mouse IgG1 | B44 | Becton-Dickinson | 347 580 | ICC |
| DCX (H-280) | Rabbit IgG | Santa Cruz | Sc-28939 | WB, ICC | |
| GABA | Rabbit IgG | Sigma | A2052 | ICC | |
| GFAP | Rabbit IgG | 6F2 | DAKO | M0761 | WB, ICC, IHC |
| GSX2 | Rabbit IgG | Abcam | ab26255 | WB | |
| HIF1α | Mouse IgG | 54/HIF-1α | Becton-Dickinson | 610 958 | WB, ICC, IHC |
| Ki67 | Mouse | MIB1 | DAKO | M7240 | ICC |
| Ki67 | Rabbit IgG | Abcam | ab15580 | ICC | |
| LEF-1 (C12A5) | Rabbit IgG | Cell Signalling | 2230 | WB | |
| LHX6 | Mouse IgG | 3D9 | Sigma | WH0026468M1 | WB |
| NG2 | Rabbit IgG | Millipore | AB5320 | ICC | |
| NKX2.1 | Rabbit IgG | EP1584Y | Abcam | ab76013 | WB |
| O4 | Mouse IgM | Gift of R. Bansal | ICC | ||
| OLIG2 | Rabbit IgG | Chemicon | ab9610 | WB, ICC | |
| S100β | Rabbit IgG | EP1576Y | Abcam | Ab52642 | WB |
| SOX2 (Y-17) | Goat IgG | Santa Cruz | Sc-17320 | WB, ICC | |
| SP8 (C-18) | Goat IgG | Santa Cruz | Sc-104661 | WB | |
| TBR1 | Rabbit IgG | Proteintech | 20932-1-AP | WB | |
| TBR2 | Rabbit IgG | Abcam | ab23345 | WB | |
| Vimentin | Mouse IgG1 | V9 | Sigma | V66630 | WB, ICC |
Abbreviations: WB, western blot; ICC, immunocytochemistry; IHC, immunohistochemistry.