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. 2016 Nov 23;26(2):354–366. doi: 10.1093/hmg/ddw392

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Published by Oxford University Press 2016. This work is written by US Government employees and is in the public domain in the US.

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Figure 3. — Establishment of a Flcn K508R mutant transgenic mouse model using BAC recombineering technology. (A) Screening for BAC transgene integration using Southern blotting. The probe detecting the chloramphenicol resistant (CMR) cassette was used for identifying BAC transgenic founder mice. (B) Sequencing result of mouse tail DNA confirming the correct mutant sequence encoding a lysine to arginine exchange. (C) Real-time PCR showing Flcn expression levels in kidneys of Flcn^f/+, CDH16-Cre control mice, Flcn ^f/d, CDH16-Cre kidney-targeted knockout mice, and Flcn ^f/d/K508R, CDH16-Cre kidney-targeted knockout mice expressing the Flcn K508R mutation. (D) Representative genotyping results for Flcn^f/+, CDH16-Cre mice and Flcn ^f/+/K508R, CDH16-Cre mice using SNP real-time PCR. N.S., no significance.