Figure 1.

Short-lived Caenorhabditis elegans mutants demonstrate deficiencies in health during development and adulthood. (A) Representative survival curves of N2 wild-type and short-lived mutants from four biological repeats (Supplementary Table 1). Survival curves were compared using Mantel-Cox log-rank tests and all short-lived mutants had significantly (p < .05) shorter median life spans than wild-type N2 (mean survival difference for daf-16(mu86) was −33%, mev-1(kn1) was −29%, hsf-1(sy441) was −37%, and sir-2.1(ok434) was −26%). (B) Mean survival at 35°C on Day 6 of adulthood was reduced in daf-16, mev-1 and hsf-1, but not in sir-2.1 (p = 8.0E-8, 2.4E-11, 2.4E-11, and 0.79, respectively, calculated using Tukey’s test). Data were pooled from three independent experiments, n = 300/strain. (C) Mean survival was reduced in all short-lived strains in response to 7.5-µM hydrogen peroxide at Day 5 of adulthood (p < .0001, Tukey’s test). Data were pooled from three independent experiments, n = 138/strain. (D) The size of selected strains based on imaged area on Day 5 of adulthood showed that only mev-1 were smaller compared with wild type (p = 2.8E-13, Tukey’s test). Data were pooled from 3 independent experiments, n = 45/strain. Data were pooled from 3 independent experiments. E) Time to development for each strain as measured from time to egg laying to first day of adulthood. Compared with wild type, mev-1 and hsf-1 had a longer development time while daf-16 and sir-2.1 were similar (p = 1E-16, p = 1.0E-16, p = .088 and p =.049, respectively Tukey’s test). Data were pooled from 4 independent experiments, n = 70/strain. F) Fecundity of each selected strain as measured by the number of eggs hatched. The mutants mev-1 and sir-2.1 had reduced hatched progeny compared with wild type (p = 1.0E-12 and 1.4E-12, respectively in Tukey’s test). Data were pooled from four independent experiments, n = 64/strain. Error bars indicate SEM. ***p < .001.