Table 3.
Detection of EBOV Genomic Material in Laboratory-generated Semen Samples and Sensitivity in Two Gene Targets using the Modified Xpert EBOV Assaya
| EBOV Concentration (PFUs/mL) |
% Positive (95% CI) Number of Positive/Total Tested |
|
|---|---|---|
| NP | GP | |
| 100 | 100 (85.8, 100) 24/24 |
95.8 (78.8, 99.9) 23/24 |
| 56 | 100 (85.8, 100) 24/24 |
70.8 (48.9, 87.4) 17/24 |
| 10 | 95.8 (78.8, 99.9) 23/24 |
25.0 (9.8, 46.7) 6/24 |
| Negative | 0 (0, 14.2) 0/24 |
0 (0, 14.2) 0/24 |
Abbreviations: CI; confidence interval; EBOV, Ebola virus; GP, glycoprotein; NP, nucleoprotein; PFUs, plaque-forming units.
aLimit of detection was observed at an EBOV concentration of 100 PFUs/mL for the GP target and 10 PFUs/mL for the NP target. No known negative sample tested (n = 24) resulted in a positive outcome.