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. 2001 Dec;12(12):3783–3796. doi: 10.1091/mbc.12.12.3783

Figure 4.

Figure 4

dsl1-7 exhibits synthetic lethal interactions with both sec20-1 and tip20-5. dsl1-7 (GWY414) was mated to sec20-1 (RSY275) (A) or tip20-5 (PC137) (B), and the resulting diploid strains were sporulated, dissected onto YPD plates, and incubated at 23°C for 2 d. Spores were then replica plated to YPD and incubated at the indicated temperatures for 2 d. Two representative tetratypes are shown. Genotypes (indicated on the right; D, DSL1; d, dsl1-7; S, SEC20, s, sec20-1; T, TIP20; t, tip20-5) were determined by complementation of the temperature sensitivity with plasmid-borne SEC20, TIP20, and/or DSL1.