Figure 5.

TSPY and TSPX differentially affect numerous canonical pathways, upstream regulators and diseases or functions in prostate cancer LNCaP cells in the presence of R1881 ligand (Supplementary Material, Tables S3–S5, respectively). Among the common ones, TSPY differentially activates most canonical pathways (Y-zScore > +2.0) associated with cell growth and proliferation and oncogenesis (A), which are either inhibited or minimally affected by TSPX. Such differences are less obvious among common upstream regulators (B) and diseases or functions (C), postulated to contribute in TSPY- or TSPX-mediated differential gene expression patterns in LNCaP cells. Notably, invasion of cells, movement and migration of tumor cells are activated, and morbidity or mortality, organismal death and apoptosis are inhibited among the cellular functions/diseases in TSPY-expressing cells; while the same functions were minimally affected in TSPX-expressing cells. X-zScore = activation z scores for TSPX-expressing cells; Y-zScore = activation z scores for TSPY-expressing cells. Ingenuity Pathway Analysis (http://www.Ingenuity.com/) was performed on July 1, 2016.