Figure 3.
α7 integrin expression is decreased in Ptrh2 null skeletal muscle. (A) Immunofluorescence and Western blot (B) of the α7 integrin showed reduced protein levels in gastrocnemius muscle in Pthr2 null (Ptrh2-/-) mice at P7 compared to WT littermate controls (n = 3 per genotype for immunoblots and immunofluorescence; bar 10 μm). (C) Western blot analysis of the gastrocnemius muscle lysate indicated that Ptrh2 and pAKT/tAKT are decreased in Ptrh2-/- and α7-/-mice compared to WT (n = 3 per genotype for immunoblots and immunofluorescence; **P<0.001). Data shown in A–C are representative of three independent experiments.
