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. 2016 Oct 25;26(1):52–64. doi: 10.1093/hmg/ddw367

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Published by Oxford University Press 2016. This work is written by US Government employees and is in the public domain in the US.

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Figure 1. — Neuronal distribution of AAV9-CamKII-GFP in the Npc1^-/- mouse brain after retro-orbital injection. (A) Excerpt from the Allen Brain Atlas, demonstrating the neuronal expression pattern of CamKII in the wildtype mouse brain. (B) Immunofluorescence of AAV9-CamKII-GFP (green) in the Npc1^-/- brain after retro-orbital injection. (C–G) Co-localization of the AAV9-CamKII-GFP signal with NeuN immunofluorescence (red), indicating incorporation of AAV9-CamKII-GFP into neuronal populations, including cortical pyramidal neurons (D, green removed in E to show double labelling, arrowheads) and CA3 hippocampal neurons (F, green removed in G to show double labelling, arrowheads).