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. 2016 Oct 25;26(1):52–64. doi: 10.1093/hmg/ddw367

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Published by Oxford University Press 2016. This work is written by US Government employees and is in the public domain in the US.

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Figure 5. — Delayed Purkinje cell death upon AAV9-CamKII-NPC1 treatment in the Npc1^-/- mouse. Immunofluorescent calbindin staining of Purkinje cells (green) in Npc1^+/+mice (A), Npc1^-/- mice without treatment (C), and Npc1^-/- mice treated with AAV9-CamKII-NPC1 (E) at 9 weeks of age [I-X in image (A) = cerebellar lobule number]. (B,D,F) Quantification of Purkinje cell number (positive cells determined by presence of staining in Purkinje cell body and dendritic arbor or axon) in posterior cerebellar lobules. All data in bar-graphs expressed as mean ± S.E.M., * P < 0.05, ** P < 0.01, one-way ANOVA with Tukey’s post-test.