Figure 6.

Dose response evaluation of putative antiepileptic drugs in scn1Lab mutant zebrafish. Putative antiepileptic compounds trazodone and lorcaserin were tested for efficacy in 5 dpf scn1Lab mutant zebrafish. Chemical structure for each compound is shown (A and B). Graphs show the change in mean velocity over five concentrations of (C) trazodone and (D) lorcaserin. Locomotion was recorded for 10 min after an exposure of 30 min (blue bars) and 90 min (yellow bars). Toxicity is indicated by dashed bars. Each bar represents the mean change in velocity ± SEM from three independent experiments. The threshold for a decrease in velocity is ≥ 40% (red line). Representative tracking plots are shown from a single experiment of six individual 5 dpf scn1Lab zebrafish at baseline and following a 30 min and 90 min exposure of 250 µM (E) trazodone or (F) lorcaserin. Total movement is shown for a 10 min recording epoch.