Figure 7.

Myc-RACK1_DE overexpression inhibits the formation of ΔNLS-TDP-43 cytoplasmic inclusions. (A) SH-SY5Y co-expressing RACK1_WT or RACK1_DE and GFP-ΔNLS-TDP-43 cDNAs were labeled with myc and TDP-43 antibodies and showed that TDP-43 positive cytoplasmic inclusions induced by the overexpression of ΔNLS-TDP-43 were reduced by the RACK1_DE expression. The arrows indicate the ΔNLS-TDP-43 cytoplasmic aggregates in RACK1_WT overexpressing cells and in cells overexpressing alone GFP-ΔNLS-TDP-43. Scale bar = 10 µm. (B) The graph shows the quantification of cells positive for DNLS-TDP-43 that present cytoplasmatic foci of TDP-43 itself. For each genotype, at least 60 cells were analysed. Statistic applied was t-test, paired, two tails (**P-value≤0.005%).