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. 2017 Sep 18;26(23):4741–4751. doi: 10.1093/hmg/ddx356

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Figure 7. — Expression and localization of wt and Glu34Lys-IFT43 in mIMCD3 cells. (A) mIMCD3 cells transfected with wt-IFT43 showed normal appearing cilia when stained with acetylated α-tubulin antibodies (red). (B) The wt-IFT43-6X-His fusion protein is localized at the tip of the cilia (green). (C) Merged image of A and B. (D) Cells transfected with Glu34Lys-IFT43 showed shortened cilia (red). (E) Co-localization of acetylated α-tubulin (red) and mut-IFT43-6X-His (green) in cells transfected with Glu34Lys-IFT43. (F) Merged image of D and E.